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KMID : 0665219890020020008
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Korean Journal of Food and Nutrition
1989 Volume.2 No. 2 p.8 ~ p.13
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Calcium Alginate-entrapped Yeast Whole-cell Invertase
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방병호/Bang, Byung Ho
이상건/양철영/Lee, Sang Gun/Yang, Cheul Young
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Abstract
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A strain of Saccharomyces cerevisiae BY-366was found to produce a strong sucrose-hydrolyzing enzyme Using this strain, the optimal culture conditions for the production of invertase were investigated. The results are as follows :
1. For enzyme production, optimal temperature, initial pH and critical concentrations of sucrose and raffinose were 30℃, 5.0 and 3.0%, respectively.
2. Enzyme production was reached maxium by organic nitrogen source, 0.3% yeast extract plus 0.5% bactopeptone.
3. It was appeared the presence of 0.1M Mn^2+ and Fe^2+ ion was essential factors, on the other hand, 0.1M Ag^+ and Hg^2+ ion almost block in yeast growth and enzyme production.
4. Invertase productivity was reached maxium within 3days on stationary culture with medium-composed of sucrose 3%, bactopeptone 0.5%, yeast extract 0.3%, K_2HPO_4 0.1%, MgSO4·7H_2O 0.05%.
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